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Result: The calibrated Light strength value is saved and stored in the permanent memory on main board. Fail : clean, reassemble and secure the UV flow cell and the connected tubing. Make sure that the detector is not exposed to e. If the test fails again, replace the flow cell and then possibly the UV Monitor, or contact a Cytiva Service Engineer. A Flow cell path length test is used to derive the actual path length of the UV flow cell. The test should be performed when the UV Monitor or the UV flow cell has been replaced, and when normalized UV absorption comparisons between different systems are needed.

The C amb and C drft values should not be altered by a user. Follow the instructions below to perform a Flow cell path length test and set a new cell length. Tap Save path length to save the value to permanent memory. Note: For higher precision, instead of using acetone, use the Fe2 SO4 3 kit, product number: This section describes how to perform pump diagnostics, calibrations and handle the pump tubing log.

Note : Before starting Diagnostics, prime the flow path with water and make sure that the outlet tubing where the pumped water is collected, is filled with demineralized water. This ensures that the volume of the collected water corresponds to the pumped volume. Note : If the collected water does not correspond to the required volume i.

After replacing the pump tubing, calibrate and diagnose the Pump again. If the collected water does not correspond to the required volume replace the Pump.

Note : Before starting the calibration, prime the flow path with water and make sure that the outlet tubing where the pumped water is collected is filled with demineralized water.

When the pump tubing is replaced, use the Reset button to reset the number of hours to 0. Follow the instructions below to check the Pump tubing log and to reset the number of hours the pump tubing has been used. The Buffer valve can be switched to allow the inlet of either buffer A or buffer B, or a mixture of A and B gradient.

Turn valve switches the valve between its two positions, for example the A and B inlet ports. This option is used for troubleshooting the valve. Enter the desired flow rate and then start the Pump by tapping Start flow , and then tap Back:Back to return to the Settings and service screen. In the Settings and service screen, tap Next to access the 2 nd screen.

There is also a click sound when the valve switches. Check that the flow is delivered from the Buffer A inlet or the Buffer B inlet, according to the selected valve position. If the valve has been replaced, tap Reset to set the Valve switches counter to 0. In the Settings and service screen, tap Next to access the 2nd screen. Note : The Sample valve is by default in the Buffer position the liquid is delivered from the Buffer inlet.

Visually inspect the flow at the outlet. If the water is not flowing properly, check the following and fix accordingly:. Note : The Wash valve is by default in Waste position the flow is diverted to waste. The Outlet valve can be switched to divert the flow, either to waste or to the Fraction collector.

Note : The Wash valve is by default in Waste valve position. Hence, the flow is diverted to Waste. This section describes how to perform Conductivity Monitor calibrations and edit the Cell constant settings. The Conductivity Monitor consists of a conductivity cell with two parallel cylindrical electrodes positioned in the flow path of the cell. One of the electrodes has a temperature sensor for measuring the temperature of the liquid in the cell.

If the flow cell has been replaced, a new cell constant value has to be set. Follow the instructions below to set the Cell constant value. In the Settings and service screen , tap Next:Next to access the 3 rd screen.

Note : For the system to recalculate the measured conductivity to conductivity at a certain reference temperature, enter the temperature in the Set ref temp field. Note : Make sure that the checkbox Enable temperature compensation in the Conductivity:Calibration screen is checked.

Note the temperature and enter it into the Set actual temp text box, and then tap Calibrate to carry out the temperature calibration. Note : Temperature compensation is enabled by default factory setting. If you want to disable temperature compensation, tap the Enable temperature compensation checkbox in the Calibration screen.

Note : Make sure that the checkbox for Enable Temperature Compensation is checked. In the Calibration screen, enter the conductivity value at the current temperature in the Set Conductivity field and then tap Calibrate to carry out the Conductivity calibration. Follow the instructions below to set the pressure to 0 when the Pressure sensor is exposed to atmospheric pressure only Zero offset. Calibration of Pressure sensor must be performed by a Cytiva Service Engineer, and must be done when the Pressure sensor has been replaced.

Disconnect the inlet tubing from the Pressure sensor to expose the sensor to atmospheric pressure only. Result : If the Fraction collector is enabled, the following Fraction collector screen opens. Tap Home test and visually observe that Frac30 rotates to home position tube no.

If the cable is not connected properly, connect the cable properly and secure it in position with a screw driver if required. If the drive sleeve has been replaced, tap Reset to set the Run time to 0. In the Settings and service screen, tap Next:Next to access the 3 rd screen. Note : If the calibration fails, repeat the test. If the calibration fails again, contact a Cytiva Service Engineer. The Log Book displays the number of hours the Instrument Display has been used.

If the Instrument Display has been replaced, it is required to reset to 0 the number of hours of display usage. If the Instrument Display has been replaced, tap Reset to set the number of hours to 0. This section describes how to handle firmware updates and export system reports. It also describes how to set delay volumes and switch valve timing. Used for optimizing the switch valve timing. It is recommended to optimize the timing of switch valve Buffer valve when wavy gradients are obtained or when fluctuations in the step gradient are observed during either system performance tests or chromatography runs.

Available options:. For exporting details on the running condition of the UV, Pump , pump tubing, all the solenoid valves and the latest 4 error messages with Error codes , to a USB memory stick.

In the Settings and service screen, tap System. Before tapping the Firmware update ensure that the system is connected to a stable source of power such as a UPS. During firmware update the system should not be switched off. Note : When the firmware update is completed, the instrument automatically restarts and displays the version of the firmware.

Follow the instructions below to export the system report to a USB memory stick. Use the system report in further contacts with Cytiva Service Engineers. Enter the internal diameter ID and length of the tubing from the Outlet valve to Frac30 in the respective fields, and then tap Save. Note : Delay volume setting needs to be set when the tube between Outlet valve and Frac30 has been replaced.

Follow the instructions below to set the Switch valve timing. If wavy gradients still are obtained, or if fluctuations on step gradient levels are large, then select Advanced timing. Set switch valve time in the range of 3. The Main board screen is reserved for operations performed by a Cytiva Service Engineer only. Do not perform any further operations from this screen. Tap Back or Home to return to the previous screen or to go to the Home screen. This section contains instructions how to disassembly, remove and replace all the modules that can be dis-assembled by the user.

Replacement of modules located on the inside of the instrument must be performed by a Cytiva Service Engineer only. If an internal part needs to be replaced, please contact a Cytiva Service Engineer.

Replacement of modules located on the wet side of the instrument must be performed by trained laboratory staff only. Note : In case of multiple valve failures, make sure that the valves are not mixed. Replace the valves one by one by removing one valve at a time from the instrument.

Required tools. Disconnect power. Always switch off power to the instrument before replacing any component on the instrument or cleaning the instrument, unless stated otherwise in the user documentation. Loosen the M3 screw from the valve using a T10 torx driver, supplied with the equipment at delivery. Note : The screw can be located on different places on the top circumference of the holder.

Replace with a new Buffer valve , connect the cable, and reconnect the tubing to the valve ports. Note : Make sure that the RJ45 connector is not moved inside the cabinet. The connector needs to be retained outside the cabinet for re-assembling of a new UV module. Place the protective cover around the flow cell to protect the electronics inside the optical unit from liquid spillage.

Note : The protective cover should be assembled after completing the assembly of the UV flow cell inside the UV Monitor by just press-fit. In the Settings and Service screen , tap UV.

Loosen the screw from the valve using a T10 torx driver, supplied with the equipment at delivery. Note : The screw can be locate on different places on the top circumference of the holder. Replace with a new Sample valve , connect the cable, and reconnect the tubing to the valve ports. Keep the pump cover open when not using the system. Open the peristaltic pump cover after you switch off the equipment.

This reduces the risk of shortening the life time of the pump tubing. Loosen the two M4 screws from the Pump using a T20 screwdriver, supplied with the equipment at delivery. Note : Make sure that the pump connector is not moved inside the cabinet. The connector needs to be retained outside the cabinet for re-assembling of a new Pump module.

Note : While loosening the screw, make sure to hold the screw in your hand before it is completely out of the Pump. There is a risk that the screw will fall into the pump head if proper care is not taken during the disassembly.

Note : Make sure that the pump cable is placed away from the fan, on the left side of the instrument after removing the Pump. This safety precautions is to ensure that the cable does not get stuck in the ventilation fan.

Note : Make sure that the pump tubing is not twisted or stretched against the rollers. Loosen the M3 screw from the Wash valve using a T10 torx driver, supplied with the equipment at delivery. Loosen the M3 screw from the Outlet valve using a T10 torx driver, supplied with the equipment at delivery. Replace with a new Outlet valve. Connect the cable, and then reconnect the tubing to the valve ports.

Note : Before replacing the valve, make sure that the part number on the new valve matches with that of the old valve Outlet valve CTVU Note : If required, twist the connector to bring the connector out of the instrument hole, before disassembly the Conductivity Monitor.

Note : Make sure that the connector is not moved inside the cabinet. The connector needs to be retained outside the cabinet for re-assembling of a new Conductivity module. Hold the Bowl assembly and orient the Bowl with the aligning ribs oriented towards the aligning groove on the Bowl holder. Note : Do not lift the fraction collector by holding the Dispenser arm. Tip : If it is hard to locate the alignment feature in corresponding parts, hold the Bowl assembly near the top of the Bowl holder and rotate the Bowl holder unless the aligning ribs gets located.

Please change the country on your profile in order to switch to another country store. Edit Profile. Product Support. In this section. Product related FAQs.

How do I unpack the system? Place the instrument on the laboratory bench. The box contains the accessories delivered with the instrument. Place the fraction collector on the laboratory bench. Remove the accessories bag located on the foam cushion. Push the Drive assembly and hold it at the retracted position.

In the same time, lift the Bowl assembly. Lower the Bowl assembly on to the Base unit. How to maintain the system? How to polish your protein with minimal efforts? How to perform purification using the stand-alone system? Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page. Sample loops. Manual injection valve. Solenoid valve. Application specific column packages. Flow path schemes.

The active flow path is depicted in Green color. Flow path details during gradient operation at specified B concentration D. Flow path details, Outlet valve collection Note : Fraction collector is disabled. Flow path details, fraction collection Note : Fraction collector is enabled. Flow path details while loading Sample through the Pump. Spare parts. Contact a Cytiva Service Engineer.

See: Troubleshooting Flow chart 5 below. The rollers are not rotating, Check the condition of the pump tubing. Check that there is no blockage on the inlet or outlet. Open the pump head and visually check if the pump rollers are rotating freely when starting the Pump from the Diagnostics screen.

If not, check if the Pump connector is connected or if anything is stuck between the rollers. Pressure sensor Error messages Error code Description Possible cause Action Overpressure Blockage in the tubing, valves or in the column.

Improperly selected Flow rate. Wrong pressure setting used for chosen application. Check tubing and valves by disconnecting one at a time starting from the Fraction collector going backwards towards the Pump. When pressure is released the blockage has been found, clean or replace tube to resolve the issue. Check the specification and pressure of the columns for correct flow rate.

Perform Zero offset. The temperature sensor is not functioning. See: Troubleshooting Flow chart 6 below. Reload Method Incomplete method.

Wrong method loaded. Reload the method. User is trying to work on features that are not supported. Restart the system. Tap Continue on the Touch screen. Make sure that there is adequate ventilation surrounding the system. Make sure that the system is not standing in direct sun light. With assistance from a Cytiva Service Engineer, check the Power supply for voltages.

Drive sleeve worn out. Sensors are not working. The Frac30 internal cable is not working. Motor failure. Main board failure. See: Troubleshooting Flow chart 4 below. Flush the UV flow cell and mount it in a secure way Or, Replace the UV flow cell if needed, and try to calibrate again. Set a lower UV light strength max counts. Tap Calibrate to get a signal at about mV.

Order a new UV Monitor. Flush the UV flow cell and mount it in a secure way. Try to recalibrate. If the voltage is still less than mV, change UV module. Error messages Error code Description Possible cause Action UV module failure During power up, too little light is reaching the detector, i.

See: Troubleshooting Flow chart 1, below. See: Troubleshooting Flow chart 2, below. Main PWA failure. See: Troubleshooting Flow chart 3, below. Preventive maintenance Preventive maintenance should be performed on a daily, weekly and monthly basis. Daily Maintenance. Visually inspect the instrument for leakages in the flow path Visually inspect the instrument for leakages in the flow path.

After use Clean the column and the system flow path after use and leave the system filled with demineralized water. Weekly, or when required. Visually inspect the inlet filters and clean them if necessary Visually inspect the inlet filters and clean them if necessary. Cleaning procedure: immerse and leave the inlet filters in 1 M NaOH for about 2 hours. Clean the system flow path Clean the system flow path with 0. Cleaning may be necessary more or less frequently, depending on the system usage and the nature of the samples.

Calibrate the pump Calibrate the Pump. When required. Clean the instrument externally Clean the instrument externally. Clean the Fraction collector Clean the Fraction collector. Clean the UV flow cell. Exei kataferei kaneis sas na kanei to FI search na doulevei swsta?? Ti ennow swsta?? Otan vazeis to VIN na sou vgazei kateu8eian to modelo me oles tis prodiagrafes kai oxi mia lista apo pi8ana matches?? Egw pantws oxi.. Ki etsi ka8e fora prepei na phgainw sto service book to autokinhtou kai na vlepw tis prodiagrafes apo ekei..

Talaipwria dhl. Sigoura exei na kanei me ta maintenance settings kai to -ini editor. Kapoios universal kwdikos xrhsth pou na se afhnei na synde8eis me internet kai na pareis oles tis plhrofories apo to VIN?? Plz send me this link to my e-mail un zipped because I can't open it from here. Pakar bahasa tubuh mengungkapnya.

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